Systematic high-content genome-wide RNAi screens of endothelial cell migration and morphology
Identifieur interne : 000137 ( Main/Exploration ); précédent : 000136; suivant : 000138Systematic high-content genome-wide RNAi screens of endothelial cell migration and morphology
Auteurs : Steven P. Williams [Australie] ; Cathryn M. Gould [Australie] ; Cameron J. Nowell [Australie] ; Tara Karnezis [Australie] ; Marc G. Achen [Australie] ; Kaylene J. Simpson [Australie] ; Steven A. Stacker [Australie]Source :
- Scientific Data [ 2052-4463 ] ; 2017.
Abstract
Many cell types undergo migration during embryogenesis and disease. Endothelial cells line blood vessels and lymphatics, which migrate during development as part of angiogenesis, lymphangiogenesis and other types of vessel remodelling. These processes are also important in wound healing, cancer metastasis and cardiovascular conditions. However, the molecular control of endothelial cell migration is poorly understood. Here, we present a dataset containing siRNA screens that identify known and novel components of signalling pathways regulating migration of lymphatic endothelial cells. These components are compared to signalling in blood vascular endothelial cells. Further, using high-content microscopy, we captured a dataset of images of migrating cells following transfection with a genome-wide siRNA library. These datasets are suitable for the identification and analysis of genes involved in endothelial cell migration and morphology, and for computational approaches to identify signalling networks controlling the migratory response and integration of cell morphology, gene function and cell signaling. This may facilitate identification of protein targets for therapeutically modulating angiogenesis and lymphangiogenesis in the context of human disease.
Url:
DOI: 10.1038/sdata.2017.9
PubMed: 28248931
PubMed Central: 5332011
Affiliations:
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Achen</name><affiliation><nlm:aff id="a1"><institution>Tumour Angiogenesis Program, Peter MacCallum Cancer Centre, 305 Grattan St, Melbourne, Victoria 3000, Australia</institution></nlm:aff></affiliation><affiliation wicri:level="1"><nlm:aff id="a2"><institution>Ludwig Institute for Cancer Research, Royal Melbourne Hospital</institution>, Parkville, Victoria 3050,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation><nlm:aff id="a5"><institution>Sir Peter MacCallum Department of Oncology, University of Melbourne, Parkville, Victoria 3010, Australia</institution></nlm:aff></affiliation></author><author><name sortKey="Simpson, Kaylene J" sort="Simpson, Kaylene J" uniqKey="Simpson K" first="Kaylene J." last="Simpson">Kaylene J. Simpson</name><affiliation wicri:level="1"><nlm:aff id="a3"><institution>Victorian Centre for Functional Genomics, Peter MacCallum Cancer Centre</institution>, 305 Grattan St, Melbourne, Victoria 3000,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation><nlm:aff id="a5"><institution>Sir Peter MacCallum Department of Oncology, University of Melbourne, Parkville, Victoria 3010, Australia</institution></nlm:aff></affiliation><affiliation wicri:level="1"><nlm:aff id="a6"><institution>Department of Pathology, University of Melbourne</institution>, Parkville, Victoria 3010,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Stacker, Steven A" sort="Stacker, Steven A" uniqKey="Stacker S" first="Steven A." last="Stacker">Steven A. Stacker</name><affiliation><nlm:aff id="a1"><institution>Tumour Angiogenesis Program, Peter MacCallum Cancer Centre, 305 Grattan St, Melbourne, Victoria 3000, Australia</institution></nlm:aff></affiliation><affiliation wicri:level="1"><nlm:aff id="a2"><institution>Ludwig Institute for Cancer Research, Royal Melbourne Hospital</institution>, Parkville, Victoria 3050,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation><nlm:aff id="a5"><institution>Sir Peter MacCallum Department of Oncology, University of Melbourne, Parkville, Victoria 3010, Australia</institution></nlm:aff></affiliation></author></analytic><series><title level="j">Scientific Data</title><idno type="eISSN">2052-4463</idno><imprint><date when="2017">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Many cell types undergo migration during embryogenesis and disease. Endothelial cells line blood vessels and lymphatics, which migrate during development as part of angiogenesis, lymphangiogenesis and other types of vessel remodelling. These processes are also important in wound healing, cancer metastasis and cardiovascular conditions. However, the molecular control of endothelial cell migration is poorly understood. Here, we present a dataset containing siRNA screens that identify known and novel components of signalling pathways regulating migration of lymphatic endothelial cells. These components are compared to signalling in blood vascular endothelial cells. Further, using high-content microscopy, we captured a dataset of images of migrating cells following transfection with a genome-wide siRNA library. These datasets are suitable for the identification and analysis of genes involved in endothelial cell migration and morphology, and for computational approaches to identify signalling networks controlling the migratory response and integration of cell morphology, gene function and cell signaling. This may facilitate identification of protein targets for therapeutically modulating angiogenesis and lymphangiogenesis in the context of human disease.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Williams, S P" uniqKey="Williams S">S. P. Williams</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Williams, S P" uniqKey="Williams S">S. P. Williams</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Williams, S P" uniqKey="Williams S">S. P. Williams</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Williams, S P" uniqKey="Williams S">S. P. Williams</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Williams, S P" uniqKey="Williams S">S. P. Williams</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Williams, S P" uniqKey="Williams S">S. P. Williams</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Australie</li></country></list><tree><country name="Australie"><noRegion><name sortKey="Williams, Steven P" sort="Williams, Steven P" uniqKey="Williams S" first="Steven P." last="Williams">Steven P. Williams</name></noRegion><name sortKey="Achen, Marc G" sort="Achen, Marc G" uniqKey="Achen M" first="Marc G." last="Achen">Marc G. Achen</name><name sortKey="Gould, Cathryn M" sort="Gould, Cathryn M" uniqKey="Gould C" first="Cathryn M." last="Gould">Cathryn M. Gould</name><name sortKey="Karnezis, Tara" sort="Karnezis, Tara" uniqKey="Karnezis T" first="Tara" last="Karnezis">Tara Karnezis</name><name sortKey="Nowell, Cameron J" sort="Nowell, Cameron J" uniqKey="Nowell C" first="Cameron J." last="Nowell">Cameron J. Nowell</name><name sortKey="Nowell, Cameron J" sort="Nowell, Cameron J" uniqKey="Nowell C" first="Cameron J." last="Nowell">Cameron J. Nowell</name><name sortKey="Simpson, Kaylene J" sort="Simpson, Kaylene J" uniqKey="Simpson K" first="Kaylene J." last="Simpson">Kaylene J. Simpson</name><name sortKey="Simpson, Kaylene J" sort="Simpson, Kaylene J" uniqKey="Simpson K" first="Kaylene J." last="Simpson">Kaylene J. Simpson</name><name sortKey="Stacker, Steven A" sort="Stacker, Steven A" uniqKey="Stacker S" first="Steven A." last="Stacker">Steven A. Stacker</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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